Application
Fig1: Sample:Liver (Mouse) Lysate at 40 ug Primary: Anti-GTP cyclohydrolase 1 (ER1910-26) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GTP cyclohydrolase 1) Polyclonal Antibody, Unconjugated (ER1910-26) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | GTP cyclohydrolase 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GTP-CH-1 DYT 5 DYT5 GCH 1 GCH GCH1 GTP CH 1 GTP CH I GTP cyclohydrolase 1 (dopa responsive dystonia) GTP cyclohydrolase 1 GTP cyclohydrolase I GTPCH 1 GTPCH1 Guanosine 5' triphosphate cyclohydrolase I DYT14 DYT5a HPABH4B. |
| Product description | Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown. |
| Immunogen | KLH conjugated synthetic peptide derived from human GTP-CH-1 34-110/250 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | GTP-CH-1 DYT 5 DYT5 GCH 1 GCH GCH1 GTP CH 1 GTP CH I GTP cyclohydrolase 1 (dopa responsive dystonia) GTP cyclohydrolase 1 GTP cyclohydrolase I GTPCH 1 GTPCH1 Guanosine 5' triphosphate cyclohydrolase I DYT14 DYT5a HPABH4B. |
|---|---|
| Molecular Weight(MW) | 27 KDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | P30793 |
|---|
Application
Fig1: Sample:Liver (Mouse) Lysate at 40 ug Primary: Anti-GTP cyclohydrolase 1 (ER1910-26) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GTP cyclohydrolase 1) Polyclonal Antibody, Unconjugated (ER1910-26) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | In epidermis, expressed predominantly in basal undifferentiated keratinocytes and in some but not all melanocytes (at protein level). |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
