Application
Fig1: Sample: HepG2 Cell (Human) Lysate at 30 ug TT Cell (Human) Lysate at 30 ug Primary: Anti-GTPBP9 (ER1910-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kDApplication
Fig2: Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti-GTPBP9 (ER1910-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kDApplication
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-GTPBP9 (ER1910-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kDApplication
Fig4: Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-GTPBP9 antibody (ER1910-28) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | GTPBP9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA damage-regulated overexpressed in cancer 45 protein DOC45 GBP45 GTP-binding protein 9 (putative) GTP-binding protein 9 GTP-binding protein PTD004 homologous yeast-44.2 protein Obg like ATPase 1 Obg-like ATPase 1 OLA1 OLA1_HUMAN PTD004. |
| Product description | Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP. |
| Immunogen | KLH conjugated synthetic peptide derived from human GTPBP9 151-250/396 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatDogCowSheepFruitfly |
| Concentration | 1mg/ml |
| Alternative Names | DNA damage-regulated overexpressed in cancer 45 protein DOC45 GBP45 GTP-binding protein 9 (putative) GTP-binding protein 9 GTP-binding protein PTD004 homologous yeast-44.2 protein Obg like ATPase 1 Obg-like ATPase 1 OLA1 OLA1_HUMAN PTD004. |
|---|---|
| Molecular Weight(MW) | 45 KDa |
| Cellular Localization | Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Predominantly cytoplasmic, shuttles between the nucleus and the cytoplasm. |
| SwissProt ID | Q9NTK5 |
|---|
Application
Fig1: Sample: HepG2 Cell (Human) Lysate at 30 ug TT Cell (Human) Lysate at 30 ug Primary: Anti-GTPBP9 (ER1910-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD
Application
Fig2: Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti-GTPBP9 (ER1910-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD
Application
Fig3: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-GTPBP9 (ER1910-28) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45kD Observed band size: 50kD
Application
Fig4: Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-GTPBP9 antibody (ER1910-28) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Expressed in all tissues tested but its expression is more abundant in testis, liver, lung, and brain. Overexpressed in several malignancies, including cancers of the colon, rectum, ovary, lung, stomach, and uterus. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
