Application
Fig1: Tissue/cell: Human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GTSE-1 Polyclonal Antibody, Unconjugated(ER1910-29) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GTSE-1) Polyclonal Antibody, Unconjugated (ER1910-29) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: Hela(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug Primary: Anti- GTSE-1 (ER1910-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 75 kD| Product Name | GTSE-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G2 and S phase-expressed protein 1 B99 B99 homolog G 2 and S phase expressed 1 G two S phase expressed protein 1 GTSE 1 GTSE-1 GTSE1 GTSE1_HUMAN. |
| Product description | May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression. |
| Immunogen | KLH conjugated synthetic peptide derived from human GTSE-1 651-739/739 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenPigCow |
| Concentration | 1mg/ml |
| Alternative Names | G2 and S phase-expressed protein 1 B99 B99 homolog G 2 and S phase expressed 1 G two S phase expressed protein 1 GTSE 1 GTSE-1 GTSE1 GTSE1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 77 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton. Note=Associated with microtubules. |
| SwissProt ID | Q9NYZ3 |
|---|
Application
Fig1: Tissue/cell: Human lung cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GTSE-1 Polyclonal Antibody, Unconjugated(ER1910-29) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GTSE-1) Polyclonal Antibody, Unconjugated (ER1910-29) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: Hela(Human) Cell Lysate at 30 ug U251(Human) Cell Lysate at 30 ug Primary: Anti- GTSE-1 (ER1910-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 75 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
