Application
Fig1: Protein: intestine(mouse) lysates at 40ug; Primary: Anti-HBP1 (ER1910-35) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 58kD Observed band size : 58kDApplication
Fig2: Sample: placenta (mouse) Lysate at 40 ug Uterus (Mouse) Lysate at 40 ug bone (Mouse) Lysate at 40 ug Primary: Anti-HBP1(ER1910-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 63 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HBP1) Polyclonal Antibody, Unconjugated (ER1910-35) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | HBP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ16340 HBP 1 HBP1_HUMAN High mobility group box transcription factor 1 HMG box containing protein 1 HMG box transcription factor 1 HMG box-containing protein 1. |
| Product description | Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4. |
| Immunogen | KLH conjugated synthetic peptide derived from human HBP1 421-514/514 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | FLJ16340 HBP 1 HBP1_HUMAN High mobility group box transcription factor 1 HMG box containing protein 1 HMG box transcription factor 1 HMG box-containing protein 1. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O60381 |
|---|
Application
Fig1: Protein: intestine(mouse) lysates at 40ug; Primary: Anti-HBP1 (ER1910-35) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 58kD Observed band size : 58kD
Application
Fig2: Sample: placenta (mouse) Lysate at 40 ug Uterus (Mouse) Lysate at 40 ug bone (Mouse) Lysate at 40 ug Primary: Anti-HBP1(ER1910-35) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 63 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HBP1) Polyclonal Antibody, Unconjugated (ER1910-35) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
