Application
Fig1: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti-HEMK2 (ER1910-43) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23kD Observed band size: 23kD| Product Name | HEMK2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C21orf127 Chromosome 21 open reading frame 127 EC 2.1.1.- HemK methyltransferase family member 2 HemK2 M.HsaHemK2P MGC19995 MTQ2 N-6 adenine-specific DNA methyltransferase 1 N-6 adenine-specific DNA methyltransferase 1 (putative) N6-DNA-methyltransferase N6AMT N6AMT1 OTTHUMP00000096393 OTTHUMP00000096394 PRED28 HEMK2_HUMAN. |
| Product description | Heterodimeric methyltransferase that catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May play a role in the modulation of arsenic-induced toxicity. May be involved in the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid. |
| Immunogen | KLH conjugated synthetic peptide derived from human HEMK2 1-100/214 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | C21orf127 Chromosome 21 open reading frame 127 EC 2.1.1.- HemK methyltransferase family member 2 HemK2 M.HsaHemK2P MGC19995 MTQ2 N-6 adenine-specific DNA methyltransferase 1 N-6 adenine-specific DNA methyltransferase 1 (putative) N6-DNA-methyltransferase N6AMT N6AMT1 OTTHUMP00000096393 OTTHUMP00000096394 PRED28 HEMK2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 23 KDa |
| SwissProt ID | Q9Y5N5 |
|---|
Application
Fig1: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti-HEMK2 (ER1910-43) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23kD Observed band size: 23kD| Positive Control | Widely expressed, with highest expression in parathyroid and pituitary glands, followed by adrenal gland and kidney, and lowest expression in leukocytes and mammary gland. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
