Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HEPACAM) Polyclonal Antibody, Unconjugated (ER1910-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- HEPACAM (ER1910-45) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 62 kD| Product Name | HEPACAM |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GlialCAM HECAM_HUMAN HEPACAM Hepatocyte cell adhesion molecule Protein hepaCAM. |
| Immunogen | KLH conjugated synthetic peptide derived from human HEPACAM 101-200/416 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | GlialCAM HECAM_HUMAN HEPACAM Hepatocyte cell adhesion molecule Protein hepaCAM. |
|---|---|
| Molecular Weight(MW) | 42 KDa |
| Cellular Localization | Cytoplasm. Membrane; Single-pass type I membrane protein; Cytoplasmic side. Note=In MCF7 breast carcinoma and hepatic Hep3B and HepG2 cell lines, localization of HEPACAM is cell density-dependent. In well spread cells, localized to punctate structures in the perinuclear membrane, cytoplasm, and at cell surface of protusions. In confluent cells, localized predominantly to the cytoplasmic membrane, particularly in areas of cell-cell contacts. Colocalizes with CDH1. |
| SwissProt ID | Q14CZ8 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HEPACAM) Polyclonal Antibody, Unconjugated (ER1910-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: HepG2(Human) Cell Lysate at 30 ug U87MG(Human) Cell Lysate at 30 ug Primary: Anti- HEPACAM (ER1910-45) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 62 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
