Application
Fig1: Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti-HERC6 (ER1910-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kDApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-HERC6 (ER1910-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (ER1910-50) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample:Brain (Mouse) Lysate at 40 ug Primary: Anti-HERC6(ER1910-50)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115kD Observed band size: 115kD| Product Name | HERC6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 6.3.2. FLJ20637 HECT domain and RCC1 like domain containing protein 6 HECT domain and RCC1-like domain-containing protein 6 Hect domain and RLD 6 HERC 6 HERC6 HERC6_HUMAN Potential ubiquitin ligase Probable E3 ubiquitin protein ligase HERC6 Probable E3 ubiquitin-protein ligase HERC6. |
| Product description | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human HERC6 751-850/1022 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigRabbit |
| Concentration | 1mg/ml |
| Alternative Names | EC 6.3.2. FLJ20637 HECT domain and RCC1 like domain containing protein 6 HECT domain and RCC1-like domain-containing protein 6 Hect domain and RLD 6 HERC 6 HERC6 HERC6_HUMAN Potential ubiquitin ligase Probable E3 ubiquitin protein ligase HERC6 Probable E3 ubiquitin-protein ligase HERC6. |
|---|---|
| Molecular Weight(MW) | 115 KDa |
| Cellular Localization | Cytoplasm, cytosol. |
| SwissProt ID | Q8IVU3 |
|---|
Application
Fig1: Sample: Embryo (Mouse) Lysate at 40 ug Primary: Anti-HERC6 (ER1910-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti-HERC6 (ER1910-50) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HERC6) Polyclonal Antibody, Unconjugated (ER1910-50) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample:Brain (Mouse) Lysate at 40 ug Primary: Anti-HERC6(ER1910-50)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115kD Observed band size: 115kD| Positive Control | Detected in brain, heart, placenta and testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
