Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated (ER1910-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug Primary: Anti-HFH4 (ER1910-53) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kDApplication
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug Primary: Anti-HFH4 (ER1910-53) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated (ER1910-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated (ER1910-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig6: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated (ER1910-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig7: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HFH4 Antibody(ER1910-53)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).| Product Name | HFH4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Forkhead box protein J1 FOXJ-1 winged helix FOXJ1 FKHL13 HFH-4 HFH4 MGC35202 forkhead box J1 Forkhead Homologue 4 Forkhead related protein FKHL13 Fox J1 Hepatocyte nuclear factor 3 forkhead homolog 4 HNF-3/forkhead homolog 4 FOXJ1_HUMAN. |
| Product description | May play an important role in cell fate determination during lung development and in spermatogenesis. |
| Immunogen | KLH conjugated synthetic peptide derived from human FOXJ1 161-260/421 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IFFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:0.2ug/test: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRatDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Forkhead box protein J1 FOXJ-1 winged helix FOXJ1 FKHL13 HFH-4 HFH4 MGC35202 forkhead box J1 Forkhead Homologue 4 Forkhead related protein FKHL13 Fox J1 Hepatocyte nuclear factor 3 forkhead homolog 4 HNF-3/forkhead homolog 4 FOXJ1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 46 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q92949 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated (ER1910-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug Primary: Anti-HFH4 (ER1910-53) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD
Application
Fig3: Sample: Testis (Mouse) Lysate at 40 ug Testis (Rat) Lysate at 40 ug Primary: Anti-HFH4 (ER1910-53) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated (ER1910-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated (ER1910-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig6: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HFH4) Polyclonal Antibody, Unconjugated (ER1910-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig7: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with HFH4 Antibody(ER1910-53)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).| Positive Control | Testis, oviduct, lung and brain cortex. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:0.2ug/test: IF:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
