Application
Fig1: Protein: Raji(human) lysates at 40ug; Primary: rabbit Anti-hHR23b (ER1910-56) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:43 kD Observed band size:50 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hHR23b) Polyclonal Antibody, Unconjugated (ER1910-56) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hHR23b) Polyclonal Antibody, Unconjugated (ER1910-56) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | hHR23b |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hHR 23b hHR23B HR 23B HR23B mHR 23B mHR23B P58 RAD 23B RAD23 (S. cerevisiae) homolog B RAD23 homolog B (S. cerevisiae) RAD23 homolog B RAD23 yeast homolog of B RAD23B RD23B_HUMAN UV excision repair protein RAD23 homolog B XP C repair complementing complex 58 kDa XP C repair complementing complex 58 kDa protein XP C repair complementing protein XP-C repair-complementing complex 58 kDa protein XPC repair complementing complex 58 kDa XPC repair complementing complex 58 kDa protein XPC repair complementing protein. |
| Product description | Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. ;Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation.;The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1. |
| Immunogen | KLH conjugated synthetic peptide derived from human hHR23b 24-120/409 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigRabbit |
| Concentration | 1mg/ml |
| Alternative Names | hHR 23b hHR23B HR 23B HR23B mHR 23B mHR23B P58 RAD 23B RAD23 (S. cerevisiae) homolog B RAD23 homolog B (S. cerevisiae) RAD23 homolog B RAD23 yeast homolog of B RAD23B RD23B_HUMAN UV excision repair protein RAD23 homolog B XP C repair complementing complex 58 kDa XP C repair complementing complex 58 kDa protein XP C repair complementing protein XP-C repair-complementing complex 58 kDa protein XPC repair complementing complex 58 kDa XPC repair complementing complex 58 kDa protein XPC repair complementing protein. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Note=The intracellular distribution is cell cycle dependent. Localized to the nucleus and the cytoplasm during G1 phase. Nuclear levels decrease during S-phase; upon entering mitosis, relocalizes in the cytoplasm without association with chromatin. |
| SwissProt ID | P54727 |
|---|
Application
Fig1: Protein: Raji(human) lysates at 40ug; Primary: rabbit Anti-hHR23b (ER1910-56) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size:43 kD Observed band size:50 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hHR23b) Polyclonal Antibody, Unconjugated (ER1910-56) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hHR23b) Polyclonal Antibody, Unconjugated (ER1910-56) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
