Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIF Prolyl Hydroxylases) Polyclonal Antibody, Unconjugated (ER1910-60) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- HIF Prolyl Hydroxylases (ER1910-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Product Name | HIF Prolyl Hydroxylases |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHD4/prolyl hydroxylase EGLN4 FLJ20262 HIF prolyl hydroxylase PH4 HIF-PH4 HIF-prolyl hydroxylase 4 HIFPH4 HPH-4 Hypoxia inducible factor prolyl 4 hydroxylase Hypoxia inducible factor prolyl hydroxylase Hypoxia-inducible factor prolyl hydroxylase 4 P4H TM P4H with transmembrane domain P4H-TM P4htm P4HTM_HUMAN PH 4 PH4 PHD4 Proline 4 hydroxylase Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) Prolyl hydroxlase domain containing 4 Transmembrane prolyl 4-hydroxylase. |
| Product description | Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. |
| Immunogen | KLH conjugated synthetic peptide derived from human PH-4 201-300/502 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | PHD4/prolyl hydroxylase EGLN4 FLJ20262 HIF prolyl hydroxylase PH4 HIF-PH4 HIF-prolyl hydroxylase 4 HIFPH4 HPH-4 Hypoxia inducible factor prolyl 4 hydroxylase Hypoxia inducible factor prolyl hydroxylase Hypoxia-inducible factor prolyl hydroxylase 4 P4H TM P4H with transmembrane domain P4H-TM P4htm P4HTM_HUMAN PH 4 PH4 PHD4 Proline 4 hydroxylase Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum) Prolyl hydroxlase domain containing 4 Transmembrane prolyl 4-hydroxylase. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type II membrane protein. |
| SwissProt ID | Q9NXG6 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIF Prolyl Hydroxylases) Polyclonal Antibody, Unconjugated (ER1910-60) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Muscle (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- HIF Prolyl Hydroxylases (ER1910-60) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD| Positive Control | Widely expressed with highest levels in adult pancreas, heart, skeletal muscle, brain, placenta, kidney and adrenal gland. Expressed at lower levels in epiphyseal cartilage and in fibroblasts. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
