Application
Fig1: Protein: lymph node(mouse) lyates at 40ug; spleen(mouse) lyates at 40ug; Primary: Rabbit Anti-HIP55 (ER1910-62) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:55 kD Observed band size:55 kD| Product Name | HIP55 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABP1 Actin-binding protein 1 Cervical mucin associated protein Cervical mucin-associated protein Cervical SH3P7 CMAP Dbnl DBNL_HUMAN Drebrin F drebrin like Drebrin like protein Drebrin-F Drebrin-like protein HIP 55 HIP-55 HPK1 interacting protein of 55 kDa HPK1-interacting protein of 55 kDa SH3 domain containing protein 7 SH3 domain-containing protein 7 SH3P7 Src Homology 3 Domain Containing Protein src homology 3 domain containing protein HIP. |
| Product description | Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin. |
| Immunogen | KLH conjugated synthetic peptide derived from human HIP55 201-300/430 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | ABP1 Actin-binding protein 1 Cervical mucin associated protein Cervical mucin-associated protein Cervical SH3P7 CMAP Dbnl DBNL_HUMAN Drebrin F drebrin like Drebrin like protein Drebrin-F Drebrin-like protein HIP 55 HIP-55 HPK1 interacting protein of 55 kDa HPK1-interacting protein of 55 kDa SH3 domain containing protein 7 SH3 domain-containing protein 7 SH3P7 Src Homology 3 Domain Containing Protein src homology 3 domain containing protein HIP. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Note=Cortical cytoskeleton. Associates with lamellipodial actin. |
| SwissProt ID | Q9UJU6 |
|---|
Application
Fig1: Protein: lymph node(mouse) lyates at 40ug; spleen(mouse) lyates at 40ug; Primary: Rabbit Anti-HIP55 (ER1910-62) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:55 kD Observed band size:55 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
