Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIPK1) Polyclonal Antibody, Unconjugated (ER1910-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Mcf-7(Human) Cell Lysate at 40 ug Primary: Anti-HIPK1(ER1910-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131kD| Product Name | HIPK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hipk 1 Hipk1 HIPK1_HUMAN Homeodomain interacting protein kinase 1 Homeodomain interacting protein kinase 1 like protein Homeodomain-interacting protein kinase 1 Myak Nbak 2 Nbak2 Nuclear body associated kinase 2 Nuclear body associated kinase 2b. |
| Product description | May play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation. |
| Immunogen | KLH conjugated synthetic peptide derived from human HIPK1 1-100/1210 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheepMacaque Monkey |
| Concentration | 1mg/ml |
| Alternative Names | Hipk 1 Hipk1 HIPK1_HUMAN Homeodomain interacting protein kinase 1 Homeodomain interacting protein kinase 1 like protein Homeodomain-interacting protein kinase 1 Myak Nbak 2 Nbak2 Nuclear body associated kinase 2 Nuclear body associated kinase 2b. |
|---|---|
| Molecular Weight(MW) | 131 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Predominantly nuclear. |
| SwissProt ID | Q86Z02 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIPK1) Polyclonal Antibody, Unconjugated (ER1910-63) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Mcf-7(Human) Cell Lysate at 40 ug Primary: Anti-HIPK1(ER1910-63) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 131 kD Observed band size: 131kD| Positive Control | Ubiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
