Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-HIRA (ER1910-64) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 112 kD Observed band size: 112 kD| Product Name | HIRA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DGCR1 DGGR 1 DGGR1 DiGeorge critical region gene 1 HIR HIR histone cell cycle regulation defective homolog A HIRA HIRA protein HIRA_HUMAN Protein HIRA TUP 1 TUP1 TUP1 like enhancer of split protein 1 TUP1-like enhancer of split protein 1 TUPLE 1 TUPLE1. |
| Product description | Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit. |
| Immunogen | KLH conjugated synthetic peptide derived from human HIRA/DGGR1 251-350/1017 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | DGCR1 DGGR 1 DGGR1 DiGeorge critical region gene 1 HIR HIR histone cell cycle regulation defective homolog A HIRA HIRA protein HIRA_HUMAN Protein HIRA TUP 1 TUP1 TUP1 like enhancer of split protein 1 TUP1-like enhancer of split protein 1 TUPLE 1 TUPLE1. |
|---|---|
| Molecular Weight(MW) | 112 KDa |
| Cellular Localization | Nucleus. Nucleus, PML body. Note=Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence. |
| SwissProt ID | P54198 |
|---|
Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-HIRA (ER1910-64) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 112 kD Observed band size: 112 kD| Positive Control | Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
