Application
Fig1: Sample: HL60(Human) Cell Lysate at 30 ug Primary: Anti-HMPH (ER1910-67) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kDApplication
Fig2: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-HMPH (ER1910-67) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Product Name | HMPH |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Hematopoietically expressed homeobox Hematopoietically-expressed homeobox protein HHEX HEX HHEX HHEX_HUMAN HMPH Homeobox protein HEX Homeobox protein PRH HOX11L PEN PRH PRHX. |
| Product description | Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human HHEX/HMPH 131-230/270 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Hematopoietically expressed homeobox Hematopoietically-expressed homeobox protein HHEX HEX HHEX HHEX_HUMAN HMPH Homeobox protein HEX Homeobox protein PRH HOX11L PEN PRH PRHX. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| Cellular Localization | Nucleus (Probable). |
| SwissProt ID | Q03014 |
|---|
Application
Fig1: Sample: HL60(Human) Cell Lysate at 30 ug Primary: Anti-HMPH (ER1910-67) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD
Application
Fig2: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-HMPH (ER1910-67) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Positive Control | Liver and promyelocytic leukemia cell line HL60. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
