Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated (ER1910-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated (ER1910-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-HNF1 (Bs-1405R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kDApplication
Fig4: Blank control:A549. Primary Antibody (green line): Rabbit Anti-HNF1A antibody (ER1910-68) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | HNF1A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HNF1A TCF1 HNF1 alpha HNF-1 Alpha Hepatocyte nuclear factor 1 Alpha Albumin proximal factor Hepatic nuclear factor 1 alpha Hepatic nuclear factor 1 Hepatic transcription factor 1 alpha Hepatic transcription factor 1 HNF 1 HNF 1A HNF1A Interferon production regulator factor LF B1 LF B1 hepatic nuclear factor LFB 1 LFB1 LFB1 hepatic nuclear factor Liver specific transcription factor LF B1 Liver specific transcription factor LFB1 Maturity onset diabetes of the young 3 MODY 3 MODY3 TCF 1 TCF1 TCF-1 Transcription factor 1 Transcription factor 1 hepatic HNF1A_HUMAN. |
| Product description | Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. |
| Immunogen | KLH conjugated synthetic peptide derived from human HNF1 201-350/628 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:3ug/test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowZebra FishSheep |
| Concentration | 1mg/ml |
| Alternative Names | HNF1A TCF1 HNF1 alpha HNF-1 Alpha Hepatocyte nuclear factor 1 Alpha Albumin proximal factor Hepatic nuclear factor 1 alpha Hepatic nuclear factor 1 Hepatic transcription factor 1 alpha Hepatic transcription factor 1 HNF 1 HNF 1A HNF1A Interferon production regulator factor LF B1 LF B1 hepatic nuclear factor LFB 1 LFB1 LFB1 hepatic nuclear factor Liver specific transcription factor LF B1 Liver specific transcription factor LFB1 Maturity onset diabetes of the young 3 MODY 3 MODY3 TCF 1 TCF1 TCF-1 Transcription factor 1 Transcription factor 1 hepatic HNF1A_HUMAN. |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Nucleus. [ALTERNATIVE PRODUCTS] Event=Alternative splicing; Named isoforms=3; Name=A; IsoId=P20823-1; Sequence=Displayed; Name=B; IsoId=P20823-2; Sequence=VSP_002250, VSP_002251; Name=C; IsoId=P20823-3; Sequence=VSP_002252, VSP_002253. |
| SwissProt ID | P15257 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated (ER1910-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HNF1A) Polyclonal Antibody, Unconjugated (ER1910-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-HNF1 (Bs-1405R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67 kD Observed band size: 67 kD
Application
Fig4: Blank control:A549. Primary Antibody (green line): Rabbit Anti-HNF1A antibody (ER1910-68) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Liver. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:3ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
