Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (ER1910-69) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (ER1910-69) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-hnRNP A2B1 (ER1910-69) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (ER1910-69) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | hnRNP A2B1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heterogeneous nuclear ribonucleoprotein A2 Heterogeneous nuclear ribonucleoprotein A2 Heterogeneous nuclear ribonucleoprotein A2/B1 Heterogeneous nuclear ribonucleoprotein A2/B1 Heterogeneous nuclear ribonucleoprotein B1 Heterogeneous nuclear ribonucleoprotein B1 Heterogeneous nuclear ribonucleoproteins A2/B1 hnRNP A2 hnRNP A2/hnRNP B1 hnRNP A2/B1 hnRNP B1 hnRNP-A2 hnRNP-B1 hnRNPA2 Hnrnpa2b1 hnRNPB1 HNRPA2B1 HNRPB1 Nuclear ribonucleoprotein particle A2 protein Nuclear ribonucleoprotein particle A2 protein RNP A2 RNP B1 RNP-A2 RNP-B1 RNPA2 RNPB1 ROA2_HUMAN SNRPB1. |
| Product description | Involved with pre-mRNA processing. Forms complexes (ribonucleosomes) with at least 20 other different hnRNP and heterogeneous nuclear RNA in the nucleus. |
| Immunogen | KLH conjugated synthetic peptide derived from human hnRNP A2B1 101-200/353 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Heterogeneous nuclear ribonucleoprotein A2 Heterogeneous nuclear ribonucleoprotein A2 Heterogeneous nuclear ribonucleoprotein A2/B1 Heterogeneous nuclear ribonucleoprotein A2/B1 Heterogeneous nuclear ribonucleoprotein B1 Heterogeneous nuclear ribonucleoprotein B1 Heterogeneous nuclear ribonucleoproteins A2/B1 hnRNP A2 hnRNP A2/hnRNP B1 hnRNP A2/B1 hnRNP B1 hnRNP-A2 hnRNP-B1 hnRNPA2 Hnrnpa2b1 hnRNPB1 HNRPA2B1 HNRPB1 Nuclear ribonucleoprotein particle A2 protein Nuclear ribonucleoprotein particle A2 protein RNP A2 RNP B1 RNP-A2 RNP-B1 RNPA2 RNPB1 ROA2_HUMAN SNRPB1. |
|---|---|
| Molecular Weight(MW) | 39 KDa |
| Cellular Localization | Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Predominantly nucleoplasmic, however isoform A2 is also found in the cytoplasm of cells in some tissues |
| SwissProt ID | P22626 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat intestine tissue ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (ER1910-69) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (ER1910-69) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-hnRNP A2B1 (ER1910-69) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 39 kD Observed band size: 39 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP A2B1) Polyclonal Antibody, Unconjugated (ER1910-69) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
