Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti- hnRNP DL (ER1910-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 48 kDApplication
min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP DL) Polyclonal Antibody, Unconjugated (ER1910-70) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | hnRNP DL |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A+U-rich element RNA binding factor AA407431 AA959857 AU rich element RNA binding factor AU-rich element RNA-binding factor D5Ertd650e D5Wsu145e Heterogeneous nuclear ribonucleoprotein D like Heterogeneous nuclear ribonucleoprotein D like protein Heterogeneous nuclear ribonucleoprotein D-like hnHNRP DL HNRDL_HUMAN HNRNP hnRNP D-like hnRNP DL HNRNPDL hnRPD like protein JKT41 binding protein JKT41-binding protein JKTBP JKTBP2 laAUF1 MGC125262 Protein laAUF1。 |
| Product description | Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes (By similarity). Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter (By similarity). Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence. |
| Immunogen | KLH conjugated synthetic peptide derived from human hnRNP DL 101-200/420 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | A+U-rich element RNA binding factor AA407431 AA959857 AU rich element RNA binding factor AU-rich element RNA-binding factor D5Ertd650e D5Wsu145e Heterogeneous nuclear ribonucleoprotein D like Heterogeneous nuclear ribonucleoprotein D like protein Heterogeneous nuclear ribonucleoprotein D-like hnHNRP DL HNRDL_HUMAN HNRNP hnRNP D-like hnRNP DL HNRNPDL hnRPD like protein JKT41 binding protein JKT41-binding protein JKTBP JKTBP2 laAUF1 MGC125262 Protein laAUF1。 |
|---|---|
| Molecular Weight(MW) | 46 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm in a TNPO1-dependent manner. |
| SwissProt ID | O14979 |
|---|
Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti- hnRNP DL (ER1910-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 48 kD
Application
min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (hnRNP DL) Polyclonal Antibody, Unconjugated (ER1910-70) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Expressed in myeloid leukemia, gastric adenocarcinoma, cervical carcinoma, hepatoma, fibrosarcoma, colon adenocarcinoma, epidermoid carcinoma, osteosarcoma and urinary bladder carcinoma cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
