Application
Fig1: Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti-HOOK1 (ER1910-72) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kD Observed band size: 85 kD| Product Name | HOOK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A930033L17Rik Abnormal spermatozoon head shape azh h-hook1 hHK1 HK1 HOOK 1 Hook homolog 1 (Drosophila) Hook1 HOOK1_HUMAN MGC10642 OTTHUMP00000010548 OTTMUSP00000008480 Protein Hook homolog 1 RP23-80B16.4. |
| Product description | Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). |
| Immunogen | KLH conjugated synthetic peptide derived from Human HOOK1 551-650/728 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | A930033L17Rik Abnormal spermatozoon head shape azh h-hook1 hHK1 HK1 HOOK 1 Hook homolog 1 (Drosophila) Hook1 HOOK1_HUMAN MGC10642 OTTHUMP00000010548 OTTMUSP00000008480 Protein Hook homolog 1 RP23-80B16.4. |
|---|---|
| Molecular Weight(MW) | 85 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm. Note=Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa. Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules. |
| SwissProt ID | Q9UJC3 |
|---|
Application
Fig1: Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti-HOOK1 (ER1910-72) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 85 kD Observed band size: 85 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
