Application
Fig1: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-HOXA6 (ER1910-73) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 26 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (ER1910-73) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (ER1910-73) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | HOXA6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HOX1B Homeo box 1B Homeo box A6 Homeobox 1B Homeobox A6 Homeobox protein Hox A6 Homeobox protein Hox-1B Homeobox protein Hox-A6 antibody Homeobox protein HoxA6 HOX 1 Hox 1B HOX1 HOX1.2 Hox1B HOXA6 HX A6 HXA6 HXA6_HUMAN. |
| Product description | Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. |
| Immunogen | KLH conjugated synthetic peptide derived from human HOXA6/HOX1B 171-233/233 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | HOX1B Homeo box 1B Homeo box A6 Homeobox 1B Homeobox A6 Homeobox protein Hox A6 Homeobox protein Hox-1B Homeobox protein Hox-A6 antibody Homeobox protein HoxA6 HOX 1 Hox 1B HOX1 HOX1.2 Hox1B HOXA6 HX A6 HXA6 HXA6_HUMAN. |
|---|---|
| Molecular Weight(MW) | 23 KDa |
| SwissProt ID | P31267 |
|---|
Application
Fig1: Sample: K562(Human) Cell Lysate at 30 ug Primary: Anti-HOXA6 (ER1910-73) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 23 kD Observed band size: 26 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (ER1910-73) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HOXA6) Polyclonal Antibody, Unconjugated (ER1910-73) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Nucleus. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
