Application
Fig1: Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-HOXB5 (ER1910-75) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kDApplication
Fig2: Blank control: Hela. Primary Antibody (green line): Rabbit Anti-HOXB5 antibody (ER1910-75) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | HOXB5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homeo box 2A Homeo box B5 Homeobox 2A Homeobox B5 Homeobox protein HHO.C10 Homeobox protein Hox B5 Homeobox protein Hox-2A Homeobox protein Hox-B5 Homeobox protein HoxB5 Homeobox protein Hu-1 Homeobox2A HomeoboxB5 HOX 2 Hox 2.1 HOX 2A HOX B5 HOX2 Hox2.1 HOX2A HOXB 5 Hoxb5 HU 1 HU1 HXB5_HUMAN. |
| Product description | Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. |
| Immunogen | KLH conjugated synthetic peptide derived from human HOXB5 181-269/269 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Homeo box 2A Homeo box B5 Homeobox 2A Homeobox B5 Homeobox protein HHO.C10 Homeobox protein Hox B5 Homeobox protein Hox-2A Homeobox protein Hox-B5 Homeobox protein HoxB5 Homeobox protein Hu-1 Homeobox2A HomeoboxB5 HOX 2 Hox 2.1 HOX 2A HOX B5 HOX2 Hox2.1 HOX2A HOXB 5 Hoxb5 HU 1 HU1 HXB5_HUMAN. |
|---|---|
| Molecular Weight(MW) | 31 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P09067 |
|---|
Application
Fig1: Sample: Spinal cord (Mouse) Lysate at 40 ug Primary: Anti-HOXB5 (ER1910-75) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD
Application
Fig2: Blank control: Hela. Primary Antibody (green line): Rabbit Anti-HOXB5 antibody (ER1910-75) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Spinal cord. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
