Application
Fig1: Sample: MCF-7(Human) Cell Lysate at 40 ug Primary: Anti-HOXD12 (ER1910-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 22 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HOXD12) Polyclonal Antibody, Unconjugated (ER1910-78) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HOXD12) Polyclonal Antibody, Unconjugated (ER1910-78) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | HOXD12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Homeobox protein Hox D12 Homeobox protein Hox-4H Homeobox protein Hox-D12 Hox 4.7, mouse, homolog of Hox 4H HOX4H HOXD12 HXD12_HUMAN. |
| Product description | Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. |
| Immunogen | KLH conjugated synthetic peptide derived from human HOXD12 171-270/270 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | Homeobox protein Hox D12
Homeobox protein Hox-4H
Homeobox protein Hox-D12
Hox 4.7 mouse homolog of Hox 4H HOX4H HOXD12 HXD12_HUMAN. |
|---|---|
| Molecular Weight(MW) | 29 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P35452 |
|---|
Application
Fig1: Sample: MCF-7(Human) Cell Lysate at 40 ug Primary: Anti-HOXD12 (ER1910-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kD Observed band size: 22 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HOXD12) Polyclonal Antibody, Unconjugated (ER1910-78) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HOXD12) Polyclonal Antibody, Unconjugated (ER1910-78) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
