Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HRG1) Polyclonal Antibody, Unconjugated (ER1910-81) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-HRG1 (ER1910-81) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HRG1) Polyclonal Antibody, Unconjugated (ER1910-81) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | HRG1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HRG-alpha HRG alpha Heregulin-alpha NRG1 type III HRG-alpha Heregulin-alpha NRG1 NRG 1 NRG-1 GGF HGL HRGA NDF SMDF Pro-neuregulin-1, membrane-bound isoform Neu differentiation factor Heregulin HRG Breast cancer cell differentiation factor p45 Acetylcholine receptor-inducing activity ARIA Sensory and motor neuron-derived factor Glial growth factor neuregulin 1 isoform HRG-alpha NRG1_HUMAN MST131 MSTP131 NRG1-IT2. |
| Product description | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. |
| Immunogen | KLH conjugated synthetic peptide derived from human Pro-neuregulin-1, membrane-bound isoform 241-340/640 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigHorseRabbitGuinea PigOther |
| Concentration | 1mg/ml |
| Alternative Names | HRG-alpha
HRG alpha
Heregulin-alpha
NRG1 type III
HRG-alpha
Heregulin-alpha
NRG1
NRG 1
NRG-1
GGF
HGL
HRGA
NDF
SMDF
Pro-neuregulin-1 membrane-bound isoform Neu differentiation factor Heregulin HRG Breast cancer cell differentiation factor p45 Acetylcholine receptor-inducing activity ARIA Sensory and motor neuron-derived factor Glial growth factor neuregulin 1 isoform HRG-alpha NRG1_HUMAN MST131 MSTP131 NRG1-IT2. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein. Note=Does not seem to be active. Neuregulin-1: Secreted. Isoform 8: Nucleus. Note=May be nuclear. Isoform 9: Secreted. Note=Has a signal peptide. Isoform 10: Membrane; Single-pass type I membrane protein. Note=May possess an internal uncleaved signal sequence. |
| SwissProt ID | Q02297 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HRG1) Polyclonal Antibody, Unconjugated (ER1910-81) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-HRG1 (ER1910-81) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HRG1) Polyclonal Antibody, Unconjugated (ER1910-81) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Type I isoforms are the predominant forms expressed in the endocardium. Isoform alpha is expressed in breast, ovary, testis, prostate, heart, skeletal muscle, lung, placenta liver, kidney, salivary gland, small intestine and brain, but not in uterus, stomach, pancreas, and spleen. Isoform 3 is the predominant form in mesenchymal cells and in non-neuronal organs, whereas isoform 6 is the major neuronal form. Isoform 8 is expressed in spinal cord and brain. Isoform 9 is the major form in skeletal muscle cells; in the nervous system it is expressed in spinal cord and brain. Also detected in adult heart, placenta, lung, liver, kidney, and pancreas. Isoform 10 is expressed in nervous system: spinal cord motor neurons, dorsal root ganglion neurons, and brain. Predominant isoform expressed in sensory and motor neurons. Not detected in adult heart, placenta, lung, liver, skeletal muscle, kidney, and pancreas. Not expressed in fetal lung, liver and kidney. Type IV isoforms are brain-specific. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
