Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-HSD17B13 (ER1910-89) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HSD17B13) Polyclonal Antibody, Unconjugated (ER1910-89) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | HSD17B13 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 17-beta-HSD 13 17-beta-hydroxysteroid dehydrogenase 13 DHB13_HUMAN HMFN0376 Hsd17b13 SCDR9 Short-chain dehydrogenase/reductase 9 UNQ497/PRO1014. |
| Product description | Highly expressed in the liver. Also detected in ovary, bone marrow, kidney, brain, lung, skeletal muscle, bladder and testis. |
| Immunogen | KLH conjugated synthetic peptide derived from human HSD17B13 171-270/300 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | 17-beta-HSD 13 17-beta-hydroxysteroid dehydrogenase 13 DHB13_HUMAN HMFN0376 Hsd17b13 SCDR9 Short-chain dehydrogenase/reductase 9 UNQ497/PRO1014. |
|---|---|
| Molecular Weight(MW) | 34 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q7Z5P4 |
|---|
Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Primary: Anti-HSD17B13 (ER1910-89) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 34 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HSD17B13) Polyclonal Antibody, Unconjugated (ER1910-89) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
