Application
Fig1: Sample: A673 Cell (Human) Lysate at 40 ug Primary: Anti-HSPA12A (ER1910-91) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kD Observed band size: 73 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HSPA12A) Polyclonal Antibody, Unconjugated (ER1910-91) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | HSPA12A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ13874 Heat shock 70 kDa protein 12A heat shock 70kD protein 12A heat shock 70kDa protein 12A HS12A_HUMAN Hspa12a KIAA0417. |
| Immunogen | KLH conjugated synthetic peptide derived from human HSPA12A 531-630/375 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:200-800: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | FLJ13874 Heat shock 70 kDa protein 12A heat shock 70kD protein 12A heat shock 70kDa protein 12A HS12A_HUMAN Hspa12a KIAA0417. |
|---|---|
| Molecular Weight(MW) | 75 KDa |
| SwissProt ID | O43301 |
|---|
Application
Fig1: Sample: A673 Cell (Human) Lysate at 40 ug Primary: Anti-HSPA12A (ER1910-91) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 75 kD Observed band size: 73 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HSPA12A) Polyclonal Antibody, Unconjugated (ER1910-91) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Widely expressed with highest levels in brain, kidney and muscle. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:200-800: ICC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
