Application
Fig1: Sample: 293T Cell(human) Lysate at 40 ug Lymph node (mouse) Lysate at 40 ug HepG2 Cell(human) Lysate at 40 ug Testis (mouse) Lysate at 40 ug Primary: Anti- HSPBAP1(ER1910-92)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 55 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated (ER1910-92) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Product Name | HSPBAP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 27 KdA heat shock protein-associated protein 1 HBAP1_HUMAN hspbap HSPB1-associated protein 1 HSPBAP1 PASS1 Protein associated with small stress protein 1 protein associating with small stress protein. |
| Product description | May play a role in cellular stress response. |
| Immunogen | KLH conjugated synthetic peptide derived from human HSPBAP1 151-250/488 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | 27 KdA heat shock protein-associated protein 1 HBAP1_HUMAN hspbap HSPB1-associated protein 1 HSPBAP1 PASS1 Protein associated with small stress protein 1 protein associating with small stress protein. |
|---|---|
| Molecular Weight(MW) | 54 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | Q96EW2 |
|---|
Application
Fig1: Sample: 293T Cell(human) Lysate at 40 ug Lymph node (mouse) Lysate at 40 ug HepG2 Cell(human) Lysate at 40 ug Testis (mouse) Lysate at 40 ug Primary: Anti- HSPBAP1(ER1910-92)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55kD Observed band size: 55 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSPBAP1) Polyclonal Antibody, Unconjugated (ER1910-92) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
