Application
Fig1: Sample: HepG2 Cell(Human)Lysate at 30 ug Primary: Anti- HSTF2 (ER1910-93)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59kDApplication
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(ER1910-93) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(ER1910-93) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | HSTF2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Heat shock factor protein 2 Heat shock transcription factor 2 HSF 2 HSF2 HSTF 2 HSF2_HUMAN. |
| Product description | DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. |
| Immunogen | KLH conjugated synthetic peptide derived from human HSTF2 401-536/536 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Heat shock factor protein 2 Heat shock transcription factor 2 HSF 2 HSF2 HSTF 2 HSF2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 59 KDa |
| Cellular Localization | Cytoplasm. Nucleus. Cytoplasmic during normal growth and moves to the nucleus upon activation. |
| SwissProt ID | Q03933 |
|---|
Application
Fig1: Sample: HepG2 Cell(Human)Lysate at 30 ug Primary: Anti- HSTF2 (ER1910-93)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59kD
Application
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(ER1910-93) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-HSTF2/HSF2 Polyclonal Antibody, Unconjugated(ER1910-93) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
