Application
Fig1: Sample: PC-3 Cell (Human) Lysate at 30 ug Primary: Anti- HTRA4 (ER1910-94) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kDApplication
Fig2: Protein: DU145(human) lyates at 30ug; Primary: Rabbit Anti-HTRA4(ER1910-94) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:49 kD Observed band size:50 kDApplication
Fig3: Protein: muscle(mouse) lyates at 30ug; Primary: Rabbit Anti-HTRA4(ER1910-94) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:49 kD Observed band size:50 kD| Product Name | HTRA4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ90724 High temperature requirement protean A4 High-temperature requirement factor A4 HTRA 4 HtrA serine peptidase 4 HTRA4 HTRA4_HUMAN Probable serine protease HTRA4 Serine protease HTRA4. |
| Product description | Serine protease. |
| Immunogen | KLH conjugated synthetic peptide derived from human HTRA4 251-350/476 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCow |
| Concentration | 1mg/ml |
| Alternative Names | FLJ90724 High temperature requirement protean A4 High-temperature requirement factor A4 HTRA 4 HtrA serine peptidase 4 HTRA4 HTRA4_HUMAN Probable serine protease HTRA4 Serine protease HTRA4. |
|---|---|
| Molecular Weight(MW) | 49 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P83105 |
|---|
Application
Fig1: Sample: PC-3 Cell (Human) Lysate at 30 ug Primary: Anti- HTRA4 (ER1910-94) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD
Application
Fig2: Protein: DU145(human) lyates at 30ug; Primary: Rabbit Anti-HTRA4(ER1910-94) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:49 kD Observed band size:50 kD
Application
Fig3: Protein: muscle(mouse) lyates at 30ug; Primary: Rabbit Anti-HTRA4(ER1910-94) at 1:300; Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000; Predicted band size:49 kD Observed band size:50 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
