Application
Fig1: Blank control: Jurkat cells(blue). Primary Antibody:Rabbit Anti-CD102 antibody(ER1910-99), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1910-99, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Molt-4(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- ICAM-2/CD102 (ER1910-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 31 kDApplication
Fig3: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-CD102 (ER1910-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD| Product Name | ICAM-2/CD102 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD102 Intercellular adhesion molecule 2 CD102 antigen ICAM 2 ICAM-2 ICAM2 Intercellular adhesion molecule 2 precursor Ly60. |
| Product description | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. |
| Immunogen | KLH conjugated synthetic peptide derived from human ICAM-2 175-277/277 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CD102 Intercellular adhesion molecule 2 CD102 antigen ICAM 2 ICAM-2 ICAM2 Intercellular adhesion molecule 2 precursor Ly60. |
|---|---|
| Molecular Weight(MW) | 28 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | P35330 |
|---|
Application
Fig1: Blank control: Jurkat cells(blue). Primary Antibody:Rabbit Anti-CD102 antibody(ER1910-99), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1910-99, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Molt-4(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti- ICAM-2/CD102 (ER1910-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 31 kD
Application
Fig3: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-CD102 (ER1910-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 28 kD Observed band size: 28 kD| Positive Control | Expressed in endothelial cells and leukocytes. High levels found in lung. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
