Application
Fig1: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-ICAM4 (ER1911-01) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kDApplication
Fig2: Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-ICAM4 (ER1911-01) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kDApplication
Fig3: Sample: placenta(Mouse) Lysate at 40 ug Primary: Anti-ICAM4 (ER1911-01) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kDApplication
Fig4: Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (ER1911-01) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | ICAM4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD242 CD242 antigen ICAM-4 Icam4 ICAM4_HUMAN Intercellular adhesion molecule 4 Landsteiner Wiener blood group glycoprotein Landsteiner-Wiener blood group glycoprotein LW LW blood group protein. |
| Product description | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM4 is also a ligand for alpha-4/beta-1 and alpha-V integrins. |
| Immunogen | KLH conjugated synthetic peptide derived from human ICAM4 85-180/271 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | CD242 CD242 antigen ICAM-4 Icam4 ICAM4_HUMAN Intercellular adhesion molecule 4 Landsteiner Wiener blood group glycoprotein Landsteiner-Wiener blood group glycoprotein LW LW blood group protein. |
|---|---|
| Molecular Weight(MW) | 27 KDa |
| Cellular Localization | Isoform Long: Cell membrane; Single-pass type I membrane protein. Isoform Short: Secreted. |
| SwissProt ID | Q14773 |
|---|
Application
Fig1: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-ICAM4 (ER1911-01) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kD
Application
Fig2: Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-ICAM4 (ER1911-01) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kD
Application
Fig3: Sample: placenta(Mouse) Lysate at 40 ug Primary: Anti-ICAM4 (ER1911-01) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 45 kD
Application
Fig4: Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Phospho-c-Fos (Ser32) antibody (ER1911-01) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Positive Control | Erythrocytes. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
