Application
Fig1: Blank controlBlue):Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-ICOS ligand antibody(ER1911-03), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-03, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-ICOS ligand (ER1911-03) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kD| Product Name | ICOS ligand |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B7 H2 B7 homolog 2 B7 homologue 2 B7 like protein Gl50 B7 related protein 1 B7-like protein Gl50 B7-related protein 1 B7-H2 B7H2 B7RP 1 B7RP-1 B7RP1 CD 275 CD275 CD275 antigen GL 50 GL50 ICOS L ICOS LG ICOS ligand ICOSL ICOSL_HUMAN Icoslg Inducible T cell co stimulator ligand KIAA0653 LICOS Transmembrane protein B7 H2 ICOS ligand ICOSLG ICOS ligand-like ICOS ligand. |
| Product description | Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. |
| Immunogen | KLH conjugated synthetic peptide derived from human ICOS ligand 19-120/302 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | B7 H2 B7 homolog 2 B7 homologue 2 B7 like protein Gl50 B7 related protein 1 B7-like protein Gl50 B7-related protein 1 B7-H2 B7H2 B7RP 1 B7RP-1 B7RP1 CD 275 CD275 CD275 antigen GL 50 GL50 ICOS L ICOS LG ICOS ligand ICOSL ICOSL_HUMAN Icoslg Inducible T cell co stimulator ligand KIAA0653 LICOS Transmembrane protein B7 H2 ICOS ligand ICOSLG ICOS ligand-like ICOS ligand. |
|---|---|
| Molecular Weight(MW) | 34 KDa |
| Cellular Localization | Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. |
| SwissProt ID | O75144 |
|---|
Application
Fig1: Blank controlBlue):Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-ICOS ligand antibody(ER1911-03), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange),used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-03, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-ICOS ligand (ER1911-03) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 35 kD| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
