Application
Fig1: Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGF I Polyclonal Antibody, Unconjugated(ER1911-10) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IGF I) Polyclonal Antibody, Unconjugated (ER1911-10) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: HepG2 (human) Lysate at 40 ug Primary: Anti-IGF1 (ER1911-10) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 7.7/21 kD Observed band size: 25 kD| Product Name | IGF I |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IBP1 IGF 1 IGF I IGF-1 IGF IA IGF IB IGF1 IGFI IGFIA Insulin like growth factor 1 (somatomedin C) Full=Insulin-like growth factor I Insulin Like Growth Factor 1 Insulin like growth factor IA Insulin like growth factor IB Mechano growth factor MGF Mechano growth factor MGF Somatomedia C Somatomedin C IGF1_HUMAN. |
| Product description | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. |
| Immunogen | KLH conjugated synthetic peptide derived from human IGF I 108-195/195 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | IBP1 IGF 1 IGF I IGF-1 IGF IA IGF IB IGF1 IGFI IGFIA Insulin like growth factor 1 (somatomedin C) Full=Insulin-like growth factor I Insulin Like Growth Factor 1 Insulin like growth factor IA Insulin like growth factor IB Mechano growth factor MGF Mechano growth factor MGF Somatomedia C Somatomedin C IGF1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 7.7/21 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P05019 |
|---|
Application
Fig1: Tissue/cell: human placenta tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGF I Polyclonal Antibody, Unconjugated(ER1911-10) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IGF I) Polyclonal Antibody, Unconjugated (ER1911-10) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: HepG2 (human) Lysate at 40 ug Primary: Anti-IGF1 (ER1911-10) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 7.7/21 kD Observed band size: 25 kD| Positive Control | Human placenta tissue, Rat brain, HepG2 Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
