Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-IGFBP2 (ER1911-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD| Product Name | IGFBP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BP 2 BP2 IBP 2 IBP-2 IBP2 IBP2_HUMAN IGF binding protein 2 IGF BP53 IGF-binding protein 2 IGFBP 2 IGFBP-2 IGFBP2 IGFBP53 Insulin like growth factor binding protein 2 36kDa Insulin like growth factor binding protein 2 Insulin like growth factor-binding protein 2 precursor Insulin-like growth factor-binding protein 2. |
| Product description | The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. pproximately 98% of IGF-1 is always bound to one of six binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. IGF-BP also binds to IGF-1 inside the liver, allowing growth hormone to continuously act upon the liver to produce more IGF-1. IGF binding proteins (IGFBPs) are proteins of 24 to 45 kDa. All six IGFBPs share 50% homology with each other and have binding affinities for IGF-I and IGF-II at the same order of magnitude as the ligands have for the IGF-IR. The IGFBPs help to lengthen the half-life of circulating IGFs in all tissues, including the prostate. Individual IGFBPs may act to enhance or attenuate IGF signaling depending on their physiological context (i.e. cell type). Even with these similarities, some characteristics are different: chromosomal location, heparin binding domains, RGD recognition site, preference for binding IGF-I or IGF-II, and glycosylation and phosphorylation differences. These structural differences can have a tremendous impact on how the IGFBPs interact with cellular basement membranes. |
| Immunogen | KLH conjugated synthetic peptide derived from human IGFBP2 101-200/305 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | BP 2 BP2 IBP 2 IBP-2 IBP2 IBP2_HUMAN IGF binding protein 2 IGF BP53 IGF-binding protein 2 IGFBP 2 IGFBP-2 IGFBP2 IGFBP53 Insulin like growth factor binding protein 2 36kDa Insulin like growth factor binding protein 2 Insulin like growth factor-binding protein 2 precursor Insulin-like growth factor-binding protein 2. |
|---|---|
| Molecular Weight(MW) | 36 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P47877 |
|---|
Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-IGFBP2 (ER1911-11) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD| Positive Control | Mouse Liver Lysate, human glioma tissue, human cervical carcinoma. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
