Application
Fig1: Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated (ER1911-13) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining.Application
Fig2: Tissue/cell: Rat adrenal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated (ER1911-13) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining.Application
Fig3: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-IGFBP4 (ER1911-13) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 27 kD| Product Name | IGFBP4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Insulin-like Growth Factor Binding Protein 4 BP 4 BP4 HT29 IGFBP IBP 4 IBP4 IGF binding protein 4 IGFBP 4 IBP4_HUMAN. |
| Product description | Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4 gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells. The protein itself does not prevent the formation of cancer. However it may reduce the growth of cancer and act as an apoptotic factor. IGFBP4 has been shown to interact with Insulin-like growth factor 1 and 2. |
| Immunogen | KLH conjugated synthetic peptide derived from human IGFBP4 181-254/254 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Insulin-like Growth Factor Binding Protein 4
BP 4 BP4 HT29 IGFBP IBP 4 IBP4 IGF binding protein 4 IGFBP 4 IBP4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 26 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P22692 |
|---|
Application
Fig1: Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated (ER1911-13) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining.
Application
Fig2: Tissue/cell: Rat adrenal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated (ER1911-13) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining.
Application
Fig3: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-IGFBP4 (ER1911-13) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 27 kD| Positive Control | Rat spleen tissue, Rat adrenal tissue, Liver (Mouse) Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
