Application
Fig1: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP5 Polyclonal Antibody, Unconjugated(ER1911-14) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP5 Polyclonal Antibody, Unconjugated(ER1911-14) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- IGFBP5 (ER1911-14) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Product Name | IGFBP5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IBP 5 IBP5 IGF binding protein 5 IGF BP5 Insulin Like Growth Factor Binding Protein 5 IBP5_HUMAN Insulin-like growth factor-binding protein 5 IBP-5 IGF-binding protein 5 IGFBP-5. |
| Product description | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
| Immunogen | KLH conjugated synthetic peptide derived from human IGFBP5 101-200/272 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | IBP 5 IBP5 IGF binding protein 5 IGF BP5 Insulin Like Growth Factor Binding Protein 5 IBP5_HUMAN Insulin-like growth factor-binding protein 5 IBP-5 IGF-binding protein 5 IGFBP-5. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P24593 |
|---|
Application
Fig1: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP5 Polyclonal Antibody, Unconjugated(ER1911-14) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IGFBP5 Polyclonal Antibody, Unconjugated(ER1911-14) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti- IGFBP5 (ER1911-14) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD| Positive Control | Osteosarcoma, and at lower levels in liver, kidney and brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
