Application
Fig1: Blank control(blue):Raji (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-IGSF8 antibody(ER1911-18), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig2: Sample: Brain(Mouse) lysate at 30ug; Primary: Anti-IGSF8 (ER1911-18) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution ; Predicted band size: 62 kD Observed band size: 55 kD| Product Name | IGSF8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD316 CD316 antigen CD81 partner 3 CD81P3 EWI-2 EWI2 Glu-Trp-Ile EWI motif-containing protein 2 igsf-8 IgSF8 IGSF8_MOUSE Immunoglobulin superfamily member 8 Immunoglobulin superfamily member 8 precursor KCT-4 KCT4 Keratinocytes-associated transmembrane protein 4 LIR-D1 PGRL Prostaglandin regulatory-like protein. |
| Product description | May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse IGSF8 101-200/611 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | CD316 CD316 antigen CD81 partner 3 CD81P3 EWI-2 EWI2 Glu-Trp-Ile EWI motif-containing protein 2 igsf-8 IgSF8 IGSF8_MOUSE Immunoglobulin superfamily member 8 Immunoglobulin superfamily member 8 precursor KCT-4 KCT4 Keratinocytes-associated transmembrane protein 4 LIR-D1 PGRL Prostaglandin regulatory-like protein. |
|---|---|
| Molecular Weight(MW) | 62 KDa |
| Cellular Localization | Cell membrane; Single-pass membrane protein. |
| SwissProt ID | Q8R366 |
|---|
Application
Fig1: Blank control(blue):Raji (fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti-IGSF8 antibody(ER1911-18), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig2: Sample: Brain(Mouse) lysate at 30ug; Primary: Anti-IGSF8 (ER1911-18) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution ; Predicted band size: 62 kD Observed band size: 55 kD| Positive Control | Expressed in lymphocytes as well as in many tissues with higher expression in brain. Detected in all regions of the brain with weak expression in the pituitary. Expressed selectively by neurons but not by glial cells. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
