Application
Fig1: Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(ER1911-19) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (ER1911-19) at 1:200 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB stainingApplication
Fig3: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-IL-10 (ER1911-19) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD| Product Name | IL-10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-10 precursor Cytokine synthesis inhibitory factor CSIF Cytokine Synthesis Inhibitory Factor IL 10 IL10A Interleukin 10 Interleukin10 MGC126450 MGC126451 TGIF IL10_HUMAN. |
| Product description | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling. |
| Immunogen | KLH conjugated synthetic peptide derived from human IL-10 101-178/178 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenPigRabbitSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | Interleukin-10 precursor
Cytokine synthesis inhibitory factor
CSIF Cytokine Synthesis Inhibitory Factor IL 10 IL10A Interleukin 10 Interleukin10 MGC126450 MGC126451 TGIF IL10_HUMAN. |
|---|---|
| Molecular Weight(MW) | 18 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P18893 |
|---|
Application
Fig1: Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-10 Polyclonal Antibody, Unconjugated(ER1911-19) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-10) Polyclonal Antibody, Unconjugated (ER1911-19) at 1:200 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining
Application
Fig3: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-IL-10 (ER1911-19) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD| Positive Control | Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
