Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (ER1911-21) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug NIH/3T3 (Mouse) CellLysate at 30 ug RAW246.7 (Mouse) CellLysate at 30 ug Primary: Anti- IL12 (ER1911-21) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 35/36 kDApplication
Fig3: Blank control (blue line): Mouse spleen (blue). Primary Antibody (green line): Rabbit Anti- IL12 antibody (ER1911-21) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (ER1911-21) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | IL12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL12 IL-12 CLMF p35 CLMF1 CTL maturation factor (TcMF) Cytotoxic lymphocyte maturation factor 1 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL 12 subunit p35 IL12A Interleukin 12 alpha chain Interleukin 12 p35 Interleukin 12A Natural killer cell stimulatory factor 1 CLMF CLMF p35 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL-12 subunit p35 IL-12A IL12 subunit p35 IL12A IL12A_HUMAN Interleukin 12 p35 Interleukin 12A Interleukin-12 subunit alpha NFSK NK cell stimulatory factor chain 1 NKSF1. NK cell stimulatory factor chain 1 NKSF1 p35 IL12A_MOUSE. |
| Product description | imer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-dependent induction of interferon gamma (INF-γ), and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse IL-12 51-150/215 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Alternative Names | IL12 IL-12 CLMF p35 CLMF1 CTL maturation factor (TcMF) Cytotoxic lymphocyte maturation factor 1 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL 12 subunit p35 IL12A Interleukin 12 alpha chain Interleukin 12 p35 Interleukin 12A Natural killer cell stimulatory factor 1 CLMF CLMF p35 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL-12 subunit p35 IL-12A IL12 subunit p35 IL12A IL12A_HUMAN Interleukin 12 p35 Interleukin 12A Interleukin-12 subunit alpha NFSK NK cell stimulatory factor chain 1 NKSF1. NK cell stimulatory factor chain 1 NKSF1 p35 IL12A_MOUSE. |
|---|---|
| Molecular Weight(MW) | 22 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P43431 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (ER1911-21) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug NIH/3T3 (Mouse) CellLysate at 30 ug RAW246.7 (Mouse) CellLysate at 30 ug Primary: Anti- IL12 (ER1911-21) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 35/36 kD
Application
Fig3: Blank control (blue line): Mouse spleen (blue). Primary Antibody (green line): Rabbit Anti- IL12 antibody (ER1911-21) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (ER1911-21) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Positive Control | Rat brain tissue, Mouse Liver Lysate, Mouse Spleen Lysate, NIH/3T3 Cell Lysate, RAW246.7 Cell Lysate, rat colitis tissue, Mouse spleen, Mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
