Application
Fig1: Sample: Liver(Rat) Lysate at 40 ug Primary: Anti-IL-12 (ER1911-22) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 24 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-12) Polyclonal Antibody, Unconjugated (ER1911-22) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | IL-12 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL12 IL-12 CLMF p35 CLMF1 CTL maturation factor (TcMF) Cytotoxic lymphocyte maturation factor 1 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL 12 subunit p35 IL12A Interleukin 12 alpha chain Interleukin 12 p35 Interleukin 12A Natural killer cell stimulatory factor 1 CLMF CLMF p35 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL-12 subunit p35 IL-12A IL12 subunit p35 IL12A IL12A_HUMAN Interleukin 12 p35 Interleukin 12A Interleukin-12 subunit alpha NFSK NK cell stimulatory factor chain 1 NKSF1. NK cell stimulatory factor chain 1 NKSF1 p35 IL12A_RAT. |
| Product description | Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. |
| Immunogen | KLH conjugated synthetic peptide derived from rat IL-12 141-200/215 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Alternative Names | IL12 IL-12 CLMF p35 CLMF1 CTL maturation factor (TcMF) Cytotoxic lymphocyte maturation factor 1 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL 12 subunit p35 IL12A Interleukin 12 alpha chain Interleukin 12 p35 Interleukin 12A Natural killer cell stimulatory factor 1 CLMF CLMF p35 Cytotoxic lymphocyte maturation factor 35 kDa subunit IL-12 subunit p35 IL-12A IL12 subunit p35 IL12A IL12A_HUMAN Interleukin 12 p35 Interleukin 12A Interleukin-12 subunit alpha NFSK NK cell stimulatory factor chain 1 NKSF1. NK cell stimulatory factor chain 1 NKSF1 p35 IL12A_RAT. |
|---|---|
| Molecular Weight(MW) | 22 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P29459 |
|---|
Application
Fig1: Sample: Liver(Rat) Lysate at 40 ug Primary: Anti-IL-12 (ER1911-22) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 24 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-12) Polyclonal Antibody, Unconjugated (ER1911-22) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
