Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-IL-13 (ER1911-23) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 32 kDApplication
Fig2: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-IL-13 (ER1911-23) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 27 kDApplication
Fig3: Sample: Molt-4(Human) Cell Lysate at 30 ug Primary: Anti-IL-13 (ER1911-23) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 32 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-13) Polyclonal Antibody, Unconjugated (ER1911-23) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | IL-13 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALRH BHR 1 BHR1 IL 13 IL13 Interleukin 13 Interleukin13 MGC116786 MGC116788 MGC116789 NC30 P600 IL13_MOUSE Interleukin-13 IL-13 T-cell activation protein P600. |
| Product description | Interleukin 13 (IL-13) is a protein that in humans is encoded by the IL13 gene. IL-13 was first cloned in 1993 and is located on chromosome 5q31 with a length of 1.4kb. It has a mass of 13 kDa and folds into 4 alpha helical bundles. The secondary structural features of IL-13 are similar to that of Interleukin 4 (IL-4); however it only has 25% sequence homology to IL-4 and is capable of IL-4 independent signaling. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, Natural killer T cell, Mast cell, Basophil cells, Eosinophil cells and Nuocyte cells. Interleukin-13 is a central regulator in IgE synthesis, goblet cell hyperplasia, mucus hypersecretion, airway hyperresponsiveness, fibrosis and chitinase up-regulation. It is a mediator of allergic inflammation and different diseases including asthma. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse IL-13 45-56/131(mo) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | ALRH
BHR 1
BHR1 IL 13 IL13 Interleukin 13 Interleukin13 MGC116786 MGC116788 MGC116789 NC30 P600 IL13_MOUSE Interleukin-13 IL-13 T-cell activation protein P600. |
|---|---|
| Molecular Weight(MW) | 12 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P20109 |
|---|
Application
Fig1: Sample: Lymph node (Mouse) Lysate at 40 ug Primary: Anti-IL-13 (ER1911-23) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 32 kD
Application
Fig2: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-IL-13 (ER1911-23) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 27 kD
Application
Fig3: Sample: Molt-4(Human) Cell Lysate at 30 ug Primary: Anti-IL-13 (ER1911-23) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12 kD Observed band size: 32 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-13) Polyclonal Antibody, Unconjugated (ER1911-23) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Lymph node (Mouse) Lysate, Jurkat(Human) Cell Lysate, Molt-4(Human) Cell Lysate, Human brain glioma. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
