Application
Fig1: Blank control (Black line): Raji (Black). Primary Antibody (green line): Rabbit Anti-IL-13Ra2 antibody (ER1911-24) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA （10min）and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Raji(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-IL-13Ra2 (ER1911-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD| Product Name | IL-13Ra2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cancer/testis antigen 19 CD213a2 CT19 I13R2_HUMAN IL-13 receptor subunit alpha-2 IL-13R subunit alpha-2 IL-13R-alpha-2 IL-13RA2 IL13 receptor IL13BP IL13R Il13ra2 Interleukin 13 binding protein Interleukin 13 receptor alpha 2 chain Interleukin-13 receptor subunit alpha-2 Interleukin-13-binding protein. |
| Product description | Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). |
| Immunogen | KLH conjugated synthetic peptide derived from human IL-13Ra2 81-180/380 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDog |
| Concentration | 1mg/ml |
| Alternative Names | Cancer/testis antigen 19 CD213a2 CT19 I13R2_HUMAN IL-13 receptor subunit alpha-2 IL-13R subunit alpha-2 IL-13R-alpha-2 IL-13RA2 IL13 receptor IL13BP IL13R Il13ra2 Interleukin 13 binding protein Interleukin 13 receptor alpha 2 chain Interleukin-13 receptor subunit alpha-2 Interleukin-13-binding protein. |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | Q14627 |
|---|
Application
Fig1: Blank control (Black line): Raji (Black). Primary Antibody (green line): Rabbit Anti-IL-13Ra2 antibody (ER1911-24) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA （10min）and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Raji(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Primary: Anti-IL-13Ra2 (ER1911-24) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 41 kD| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
