Application
Fig1: Sample: Lane1: Lung(Rat) Lysate at 30 ug Lane2: Pancreas(Rat) Lysate at 30 ug Primary: Anti-IL-17C (ER1911-25) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 20kD Observed band size : 22kDApplication
Fig2: Tissue/cell: Human colon cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL17C Polyclonal Antibody, Unconjugated(ER1911-25) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL17C) Polyclonal Antibody, Unconjugated (ER1911-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | IL17C |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-17C IL17C CX2 IL-17C IL 17C IL-21 IL 21 MGC126884 MGC138401 Cytokine CX2 IL17C_HUMAN. |
| Product description | Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. |
| Immunogen | KLH conjugated synthetic peptide derived from human IL-17C 101-197/197 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Interleukin-17C IL17C CX2 IL-17C IL 17C IL-21 IL 21 MGC126884 MGC138401 Cytokine CX2 IL17C_HUMAN. |
|---|---|
| Molecular Weight(MW) | 20 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q9P0M4 |
|---|
Application
Fig1: Sample: Lane1: Lung(Rat) Lysate at 30 ug Lane2: Pancreas(Rat) Lysate at 30 ug Primary: Anti-IL-17C (ER1911-25) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution; Predicted band size : 20kD Observed band size : 22kD
Application
Fig2: Tissue/cell: Human colon cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL17C Polyclonal Antibody, Unconjugated(ER1911-25) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL17C) Polyclonal Antibody, Unconjugated (ER1911-25) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
