Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-1R2) Polyclonal Antibody, Unconjugated (ER1911-27) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Blank control: RSC96 cells(blue). Primary Antibody: Rabbit Anti-IL-1R2 antibody(ER1911-27), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.Application
Fig3: Sample: SW480 Cell (Human) Lysate at 40 ug Primary: Anti- IL-1R2 (ER1911-27) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD| Product Name | IL-1R2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL1 Receptor II IL-1R II IL1 Receptor 2 interleukin-1 receptor type 2 precursor IL1R2 CD121b IL1RB CD121 CD121 antigen-like family member B CD121b CD121B Antigen CDW121B IL-1R-2 IL-1R-beta IL-1R2 IL-1RT-2 IL-1RT2 IL1R Beta Il1r-2 IL1R2 IL1RB Interleukin 1 receptor, beta, type II Interleukin 1 receptor, type II Interleukin-1 receptor beta Interleukin-1 receptor type 2 MGC137383 MGC47725 p68 IL1 Receptor II IL1R2_HUMAN. |
| Product description | Non-signaling receptor for IL1A, IL1B and IL1RN. Reduces IL1B activities. Serves as a decoy receptor by competetive binding to IL1B and preventing its binding to IL1R1. Also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. |
| Immunogen | KLH conjugated synthetic peptide derived from human IL-1R II 301-398/398 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg/test: |
|
| Species Reactivity | HumanMouseRatDog |
| Concentration | 1mg/ml |
| Alternative Names | IL1 Receptor II
IL-1R II
IL1 Receptor 2
interleukin-1 receptor type 2 precursor
IL1R2
CD121b
IL1RB
CD121
CD121 antigen-like family member B
CD121b
CD121B Antigen
CDW121B
IL-1R-2
IL-1R-beta
IL-1R2
IL-1RT-2
IL-1RT2
IL1R Beta
Il1r-2
IL1R2
IL1RB
Interleukin 1 receptor beta type II Interleukin 1 receptor type II Interleukin-1 receptor beta Interleukin-1 receptor type 2 MGC137383 MGC47725 p68 IL1 Receptor II IL1R2_HUMAN. |
|---|---|
| Molecular Weight(MW) | 44 KDa |
| Cellular Localization | Isoform Short: Secreted. ;Isoform Long: Cell membrane; Single-pass type I membrane protein. |
| SwissProt ID | P27930 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-1R2) Polyclonal Antibody, Unconjugated (ER1911-27) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Blank control: RSC96 cells(blue). Primary Antibody: Rabbit Anti-IL-1R2 antibody(ER1911-27), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Application
Fig3: Sample: SW480 Cell (Human) Lysate at 40 ug Primary: Anti- IL-1R2 (ER1911-27) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 44 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg/test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
