Application
Fig1: Tissue/cell: Human oral squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-1RA Polyclonal Antibody, Unconjugated(ER1911-28) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Lane1:Plasma(Mouse) Tissue Lysate at 30 ug Lane2:Hemocyte(Mouse) Tissue Lysate at 30 ug Primary: Anti-IL-1RA (ER1911-28)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 23 kDApplication
Fig3: Sample: Spleen(Mouse) Tissue Lysate at 30 ug Primary: Anti-IL-1RA (ER1911-28)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 23 kDApplication
Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-1RA) Polyclonal Antibody, Unconjugated (ER1911-28) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | IL-1RA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-1 receptor antagonist ICIL 1RA IL1 inhibitor ICIL1RA IL1F3 IL1RN IRAP IL1RA_HUMAN. |
| Product description | Inhibits the activity of interleukin-1 by binding to eceptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. |
| Immunogen | KLH conjugated synthetic peptide derived from human IL-1RA 26-120/178 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowSheep |
| Concentration | 1mg/ml |
| Alternative Names | Interleukin-1 receptor antagonist ICIL 1RA IL1 inhibitor ICIL1RA IL1F3 IL1RN IRAP IL1RA_HUMAN. |
|---|---|
| Molecular Weight(MW) | 17 KDa |
| Cellular Localization | Isoform 1: Secreted. ;Isoform 2, 3, 4: Cytoplasm. |
| SwissProt ID | P25085 |
|---|
Application
Fig1: Tissue/cell: Human oral squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-1RA Polyclonal Antibody, Unconjugated(ER1911-28) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Lane1:Plasma(Mouse) Tissue Lysate at 30 ug Lane2:Hemocyte(Mouse) Tissue Lysate at 30 ug Primary: Anti-IL-1RA (ER1911-28)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 23 kD
Application
Fig3: Sample: Spleen(Mouse) Tissue Lysate at 30 ug Primary: Anti-IL-1RA (ER1911-28)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 23 kD
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (mouse spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-1RA) Polyclonal Antibody, Unconjugated (ER1911-28) at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | The intracellular form of IL1RN is predominantly expressed in epithelial cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
