Application
Fig1: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-IL2 Receptor beta (ER1911-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kDApplication
Fig2: Blank control: U937 (blue). Primary Antibody:Rabbit Anti-IL2 Receptor beta antibody(ER1911-29), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-29, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL2 Receptor beta) Polyclonal Antibody, Unconjugated (ER1911-29) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | IL2 Receptor beta |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Imterleukm-2 Receptor beta CD 122 CD122 CD122 antigen High affinity IL 2 receptor beta subunit High affinity IL 2 receptor subunit beta High affinity IL2 receptor beta subunit High affinity IL2 receptor subunit beta High affinity IL-2 receptor subunit beta IL 2 receptor IL-2 receptor IL-2 receptor subunit beta IL-2R subunit beta IL-2RB IL15RB IL2 R B IL-2 R B IL2 RB IL2 receptor IL2RB Interleukin 2 receptor beta Interleukin 2 receptor subunit beta Interleukin-2 receptor subunit beta OTTHUMP00000028799 P70 75 P7075 p75 IL2RB_HUMAN. |
| Product description | Interleukin-2 receptor subunit beta is a protein that in humans is encoded by the IL2RB gene. Also known as CD122; IL15RB; P70-75. The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit (also called CD25) and is not involved in signal transduction. The intermediate affinity form consists of a gamma/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. This protein also forms one of the three subunits of the IL-15 receptor. Activation of the receptor increases proliferation of CD8+ effector T cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human CD122 501-551/551 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Imterleukm-2 Receptor beta
CD 122
CD122 CD122 antigen High affinity IL 2 receptor beta subunit High affinity IL 2 receptor subunit beta High affinity IL2 receptor beta subunit High affinity IL2 receptor subunit beta High affinity IL-2 receptor subunit beta IL 2 receptor IL-2 receptor IL-2 receptor subunit beta IL-2R subunit beta IL-2RB IL15RB IL2 R B IL-2 R B IL2 RB IL2 receptor IL2RB Interleukin 2 receptor beta Interleukin 2 receptor subunit beta Interleukin-2 receptor subunit beta OTTHUMP00000028799 P70 75 P7075 p75 IL2RB_HUMAN. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | P14784 |
|---|
Application
Fig1: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-IL2 Receptor beta (ER1911-29) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD
Application
Fig2: Blank control: U937 (blue). Primary Antibody:Rabbit Anti-IL2 Receptor beta antibody(ER1911-29), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-29, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL2 Receptor beta) Polyclonal Antibody, Unconjugated (ER1911-29) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | Raji(Human) Cell Lysate, U937 cell, human lung carcinoma. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
