Application
Fig1: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-IL22 (ER1911-31) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 19 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL22) Polyclonal Antibody, Unconjugated (ER1911-31) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Blank control(blue): Mouse liver cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-IL22 antibody(ER1911-31), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | IL22 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-22 Il22 IL-22 Iltif ILTIFa MGC129416 MGC129417 IL-10-related T-cell-derived-inducible factor IL-TIF IL-TIF alpha Interleukin-22a IL-22a IL 21 IL D110 IL TIF IL21 IL22 ILD110 interleukin 21 MGC79382 MGC79384 TIFa TIFIL 23 TIFIL23 zcyto18. |
| Product description | Cytokine that contributes to the inflammatory response in vivo. |
| Immunogen | KLH conjugated synthetic peptide derived from human IL-22 35-179/179 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatDog |
| Concentration | 1mg/ml |
| Alternative Names | Interleukin-22 Il22 IL-22 Iltif ILTIFa MGC129416 MGC129417 IL-10-related T-cell-derived-inducible factor IL-TIF IL-TIF alpha Interleukin-22a IL-22a IL 21 IL D110 IL TIF IL21 IL22 ILD110 interleukin 21 MGC79382 MGC79384 TIFa TIFIL 23 TIFIL23 zcyto18. |
|---|---|
| Molecular Weight(MW) | 17 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q9GZX6 |
|---|
Application
Fig1: Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-IL22 (ER1911-31) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 19 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL22) Polyclonal Antibody, Unconjugated (ER1911-31) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Blank control(blue): Mouse liver cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-IL22 antibody(ER1911-31), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
