Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-IL33 (ER1911-36) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 25 kDApplication
Fig2: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-IL33 (ER1911-36) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 25 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (human heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (ER1911-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample: 293T(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-IL33 (ER1911-36) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kDApplication
Fig5: Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (ER1911-36) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Product Name | IL33 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IL-33 Interleukin-33/Nuclear factor from high endothelial venules IL33 C9orf26 CHROMOSOME 9 OPEN READING FRAME 26 DKFZp586H0523 DVS27 DVS27 related protein IL 1F11 IL 33 IL1F11 Interleukin 1 family member 11 Interleukin 33 INTERLEUKIN 33 NFHEV Interleukin 33 precursor Interleukin33 NF HEV NFEHEV NFHEV Nuclear factor from high endothelial venules RP11 575C20.2 IL33_HUMAN. |
| Product description | Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Acts as a chemoattractant tor Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury. ;In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. |
| Immunogen | KLH conjugated synthetic peptide derived from human IL-33 Propeptide 1-111/270 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | IL-33 Interleukin-33/Nuclear factor from high endothelial venules IL33 C9orf26 CHROMOSOME 9 OPEN READING FRAME 26 DKFZp586H0523 DVS27 DVS27 related protein IL 1F11 IL 33 IL1F11 Interleukin 1 family member 11 Interleukin 33 INTERLEUKIN 33 NFHEV Interleukin 33 precursor Interleukin33 NF HEV NFEHEV NFHEV Nuclear factor from high endothelial venules RP11 575C20.2 IL33_HUMAN. |
|---|---|
| Molecular Weight(MW) | 31 KDa |
| Cellular Localization | Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Note=Associates with heterochromatin and mitotic chromosomes. Translocation from the nucleus occurs upon biomechanical strain, depends on an intact microtubule network, and is ATP-dependent. |
| SwissProt ID | O95760 |
|---|
Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-IL33 (ER1911-36) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 25 kD
Application
Fig2: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-IL33 (ER1911-36) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 25 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (human heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (ER1911-36) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample: 293T(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-IL33 (ER1911-36) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31 kD Observed band size: 31 kD
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (IL33) Polyclonal Antibody, Unconjugated (ER1911-36) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.| Positive Control | Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
