Application
Fig1: Sample: A431(Human) Cell Lysate at 30 ug U-87MG(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-IL-4R (ER1911-37) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 118 kDApplication
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- IL-4R (ER1911-37)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88kD Observed band size: 118kDApplication
Fig3: Blank control: Raji (blue). Primary Antibody:Rabbit Anti-IL-4R antibody(ER1911-37), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-37, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | IL-4R |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-4 Receptor 582J2.1 CD124 CD-124 CD 124 IL 4R alpha IL4RA Interleukin 4 receptor alpha chain MGC118473. |
| Product description | Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. ;Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human IL-4R 201-300/825 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:500-2000: FC:1μg /test: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRatPigHorse |
| Concentration | 1mg/ml |
| Alternative Names | Interleukin-4 Receptor 582J2.1 CD124 CD-124 CD 124 IL 4R alpha IL4RA Interleukin 4 receptor alpha chain MGC118473. |
|---|---|
| Molecular Weight(MW) | 88 KDa |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. |
| SwissProt ID | P24394 |
|---|
Application
Fig1: Sample: A431(Human) Cell Lysate at 30 ug U-87MG(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-IL-4R (ER1911-37) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88 kD Observed band size: 118 kD
Application
Fig2: Sample: Testis (Mouse) Lysate at 40 ug Primary: Anti- IL-4R (ER1911-37)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 88kD Observed band size: 118kD
Application
Fig3: Blank control: Raji (blue). Primary Antibody:Rabbit Anti-IL-4R antibody(ER1911-37), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-37, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Positive Control | Isoform 1 and isoform 2 are highly expressed in activated T-cells. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: ICC:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
