Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (ER1911-38) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (ER1911-38) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Sample: Lovo Cell (Human) Lysate at 30 ug Primary: Anti- IL-5 (ER1911-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13kD Observed band size: 10kD| Product Name | IL-5 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | B Cell Differentiation Factor I IL5 IL 5 Colony stimulating factor Colony Stimulating Factor Eosinophil EDF Eosinophil Eosinophil Differentiation Factor IL 5 Interleukin 5 B-cell differentiation factor I IL-5 IL5_RAT Interleukin-5 T Cell Replacing Factor T-cell replacing factor Interleukin5 TRF. |
| Product description | Through binding to the interleukin-5 receptor, interleukin 5 stimulates B cell growth and increases immunoglobulin secretion - primarily IgA. It is also a key mediator in eosinophil activation. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely interleukin 3 and GM-CSF), this glycoprotein in its active form is a homodimer. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma, wherein a large increase in the number of circulating, airway tissue, and induced sputum eosinophils have been observed. Given the high concordance of eosinophils and, in particular, allergic asthma pathology, it has been widely speculated that eosinophils have an important role in the pathology of this disease. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse IL-5 56-132/132 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | B Cell Differentiation Factor I
IL5
IL 5 Colony stimulating factor Colony Stimulating Factor Eosinophil EDF Eosinophil Eosinophil Differentiation Factor IL 5 Interleukin 5 B-cell differentiation factor I IL-5 IL5_RAT Interleukin-5 T Cell Replacing Factor T-cell replacing factor Interleukin5 TRF. |
|---|---|
| Molecular Weight(MW) | 13 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P04401 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (ER1911-38) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (ER1911-38) at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Sample: Lovo Cell (Human) Lysate at 30 ug Primary: Anti- IL-5 (ER1911-38)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 13kD Observed band size: 10kD| Positive Control | Rat kidney, Rat kidney, Lovo Cell (Human) Lysate. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:100-500: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
