Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (ER1911-41) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-IL-9 (ER1911-41) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kD Observed band size: 16 kDApplication
Fig3: Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-9 Polyclonal Antibody, Unconjugated(ER1911-41) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | IL-9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Interleukin-9 IL9 Homolog of mouse T cell and mast cell growth factor 40 HP40 IL 9 Mast cell growth factor MCGF Megakaryoblast growth factor p40 cytokine p40 T cell and mast cell growth factor p40 T-cell and mast cell growth factor T cell growth factor 3 T cell growth factor p40 T-cell growth factor p40 Interleukin 9 TCGF 3. |
| Product description | Supports IL-2 independent and IL-4 independent growth of helper T-cells. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse IL-9 21-120/144 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IF |
| WB:1:500-2000: IHC-P:1:400-800: IF:1:200-800: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Interleukin-9 IL9 Homolog of mouse T cell and mast cell growth factor 40 HP40 IL 9 Mast cell growth factor MCGF Megakaryoblast growth factor p40 cytokine p40 T cell and mast cell growth factor p40 T-cell and mast cell growth factor T cell growth factor 3 T cell growth factor p40 T-cell growth factor p40 Interleukin 9 TCGF 3. |
|---|---|
| Molecular Weight(MW) | 16 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P15247 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-9) Polyclonal Antibody, Unconjugated (ER1911-41) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti-IL-9 (ER1911-41) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kD Observed band size: 16 kD
Application
Fig3: Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-9 Polyclonal Antibody, Unconjugated(ER1911-41) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Application Notes | WB:1:500-2000: IHC-P:1:400-800: IF:1:200-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
