Application
Fig1: Blank control: A431. Primary Antibody (green line): Rabbit Anti-ILDR1 antibody (ER1911-42) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti-ILDR1 (ER1911-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 56 kDApplication
Fig3: Sample: Prostate(Rat) Cell Lysate at 40 ug Primary: Anti-ILDR1 (ER1911-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 56 kDApplication
Fig4: Sample: Pancreas(Mouse) Cell Lysate at 40 ug Primary: Anti-ILDR1 (ER1911-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 56kD| Product Name | ILDR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ILDR1alpha ILDR1beta Immunoglobulin like domain containing receptor 1 alpha Immunoglobulin like domain containing receptor 1 alpha Immunoglobulin like domain containing receptor 1 Immunoglobulin like domain containing receptor 1 beta MGC50831 ILDR1_HUMAN. |
| Product description | Putative membrane receptor. |
| Immunogen | KLH conjugated synthetic peptide derived from human ILDR1 101-200/546 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | ILDR1alpha ILDR1beta Immunoglobulin like domain containing receptor 1 alpha Immunoglobulin like domain containing receptor 1 alpha Immunoglobulin like domain containing receptor 1 Immunoglobulin like domain containing receptor 1 beta MGC50831 ILDR1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 60 KDa |
| Cellular Localization | Cell membrane; Single pass type I membrane protein. Isoform 5: Cytoplasm (cytosol). |
| SwissProt ID | Q86SU0 |
|---|
Application
Fig1: Blank control: A431. Primary Antibody (green line): Rabbit Anti-ILDR1 antibody (ER1911-42) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at -20℃ .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: DU145(Human) Cell Lysate at 30 ug Primary: Anti-ILDR1 (ER1911-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 56 kD
Application
Fig3: Sample: Prostate(Rat) Cell Lysate at 40 ug Primary: Anti-ILDR1 (ER1911-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 56 kD
Application
Fig4: Sample: Pancreas(Mouse) Cell Lysate at 40 ug Primary: Anti-ILDR1 (ER1911-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 60 kD Observed band size: 56kD| Positive Control | Mainly expressed in prostate and to a lower extent in testis, pancreas, kidney, heart and liver. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
